Primer Details for TRINITY_DN5574_c0_g1_i1 |
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| (TA)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGATGGTCTATTAATGGGGAAGGA | TGCGGTTTGATTTGATGGCA | Start: 293 |
| Tm | 59.927 | 59.035 | End: 425 |
| Size | 25 | 20 | 133 |
| Primer 2 details |
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| Sequence | AGTGATGGTCTATTAATGGGGAAGG | TGCGGTTTGATTTGATGGCA | Start: 292 |
| Tm | 59.926 | 59.035 | End: 425 |
| Size | 25 | 20 | 134 |
| Primer 3 details |
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| Sequence | TGATGGTCTATTAATGGGGAAGGA | TGCGGTTTGATTTGATGGCA | Start: 294 |
| Tm | 58.768 | 59.035 | End: 425 |
| Size | 24 | 20 | 132 |
| Primer 4 details |
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| Sequence | GTGTGTTTGATATGTTGTGGAGACA | TGCGGTTTGATTTGATGGCA | Start: 248 |
| Tm | 59.762 | 59.035 | End: 425 |
| Size | 25 | 20 | 178 |
| Primer 5 details |
|---|
| Sequence | GTGATGGTCTATTAATGGGGAAGG | TGCGGTTTGATTTGATGGCA | Start: 293 |
| Tm | 58.683 | 59.035 | End: 425 |
| Size | 24 | 20 | 133 |
