ssr_primer
Primer Details for TRINITY_DN5549_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTCCCCACTACATGCCCC | ACTTGTCCCCAAACCACAGG | Start: 146 |
| Tm | 59.96 | 60.106 | End: 322 |
| Size | 20 | 20 | 177 |
| Primer 2 details |
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| Sequence | TTTTCCCCACTACATGCCCC | AAGTGCATTGGAAGGAGCCA | Start: 146 |
| Tm | 59.96 | 59.887 | End: 380 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
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| Sequence | TGGCTCCTTCCAATGCACTT | GGTGAGGTGAGAAGAGGCAG | Start: 361 |
| Tm | 59.887 | 59.751 | End: 544 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
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| Sequence | TGGCTCCTTCCAATGCACTT | CTGGTGAGGTGAGAAGAGGC | Start: 361 |
| Tm | 59.887 | 59.751 | End: 546 |
| Size | 20 | 20 | 186 |
| Primer 5 details |
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| Sequence | TTTTCCCCACTACATGCCCC | AGTGCATTGGAAGGAGCCAG | Start: 146 |
| Tm | 59.96 | 60.323 | End: 379 |
| Size | 20 | 20 | 234 |
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