ssr_primer
Primer Details for TRINITY_DN5548_c0_g1_i1 |
|---|
| (GA)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCCATGGTCACCTGAAGAA | TCGTTGTCCGTTCTTCCTGG | Start: 93 |
| Tm | 59.962 | 59.967 | End: 360 |
| Size | 20 | 20 | 268 |
| Primer 2 details |
|---|
| Sequence | GGCCATGGTCACCTGAAGAA | TCTTCCTGGTAACTGCGCTG | Start: 93 |
| Tm | 59.962 | 60.037 | End: 349 |
| Size | 20 | 20 | 257 |
| Primer 3 details |
|---|
| Sequence | GGCCATGGTCACCTGAAGAA | CTGCGCTGCAATTATCGACC | Start: 93 |
| Tm | 59.962 | 60.042 | End: 337 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | CAGCGCAGTTACCAGGAAGA | TGTAGTTGCCGTGCGATGAT | Start: 330 |
| Tm | 60.037 | 60.108 | End: 461 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
|---|
| Sequence | GGTCGATAATTGCAGCGCAG | TGTAGTTGCCGTGCGATGAT | Start: 318 |
| Tm | 60.042 | 60.108 | End: 461 |
| Size | 20 | 20 | 144 |
(GA)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCCATGGTCACCTGAAGAA | TCGTTGTCCGTTCTTCCTGG | Start: 93 |
| Tm | 59.962 | 59.967 | End: 360 |
| Size | 20 | 20 | 268 |
| Primer 2 details |
|---|
| Sequence | GGCCATGGTCACCTGAAGAA | TCTTCCTGGTAACTGCGCTG | Start: 93 |
| Tm | 59.962 | 60.037 | End: 349 |
| Size | 20 | 20 | 257 |
| Primer 3 details |
|---|
| Sequence | GGCCATGGTCACCTGAAGAA | CTGCGCTGCAATTATCGACC | Start: 93 |
| Tm | 59.962 | 60.042 | End: 337 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | CAGCGCAGTTACCAGGAAGA | TGTAGTTGCCGTGCGATGAT | Start: 330 |
| Tm | 60.037 | 60.108 | End: 461 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
|---|
| Sequence | GGTCGATAATTGCAGCGCAG | TGTAGTTGCCGTGCGATGAT | Start: 318 |
| Tm | 60.042 | 60.108 | End: 461 |
| Size | 20 | 20 | 144 |
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