ssr_primer
Primer Details for TRINITY_DN5539_c0_g1_i1 |
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| (T)10aatcaaagagataaaaaataataataataaaagaaaaaggaaaaatccaaaac(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCCCTTTAGTAACCGACCC | TTTGTGGCCGGTTTTTGACC | Start: 274 |
| Tm | 60.108 | 59.824 | End: 444 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
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| Sequence | GCCCCTTTAGTAACCGACCC | AGGGCGTGACGGTAAATGAA | Start: 274 |
| Tm | 60.108 | 59.678 | End: 513 |
| Size | 20 | 20 | 240 |
| Primer 3 details |
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| Sequence | GCCCCTTTAGTAACCGACCC | AAGGGCGTGACGGTAAATGA | Start: 274 |
| Tm | 60.108 | 59.678 | End: 514 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
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| Sequence | TAACCGACCCTACCCACTCC | TTTGTGGCCGGTTTTTGACC | Start: 284 |
| Tm | 60.325 | 59.824 | End: 444 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
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| Sequence | AGTAACCGACCCTACCCACT | TTTGTGGCCGGTTTTTGACC | Start: 282 |
| Tm | 59.589 | 59.824 | End: 444 |
| Size | 20 | 20 | 163 |
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