ssr_primer
Primer Details for TRINITY_DN5523_c0_g2_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCCTTACTATTGTAGGGACTG | AAGAAGAGAGGGGAGAGGGG | Start: 166 |
| Tm | 59.29 | 59.658 | End: 294 |
| Size | 23 | 20 | 129 |
| Primer 2 details |
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| Sequence | CCCCTCTCCCCTCTCTTCTT | GGATGGAAATCATTCGTACTGCA | Start: 275 |
| Tm | 59.658 | 59.123 | End: 523 |
| Size | 20 | 23 | 249 |
| Primer 3 details |
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| Sequence | CCCCTCTCCCCTCTCTTCTT | TGGATGGAAATCATTCGTACTGC | Start: 275 |
| Tm | 59.658 | 59.123 | End: 524 |
| Size | 20 | 23 | 250 |
| Primer 4 details |
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| Sequence | GCCCTTACTATTGTAGGGACTGA | AAGAAGAGAGGGGAGAGGGG | Start: 167 |
| Tm | 59.035 | 59.658 | End: 294 |
| Size | 23 | 20 | 128 |
| Primer 5 details |
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| Sequence | TGCCCTTACTATTGTAGGGACT | AAGAAGAGAGGGGAGAGGGG | Start: 166 |
| Tm | 57.996 | 59.658 | End: 294 |
| Size | 22 | 20 | 129 |
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