Primer Details for TRINITY_DN5485_c0_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGCCGATTGTGGTCTACTGA | TCGCACTCCAAATTTAAGAAAATTGT | Start: 239 |
| Tm | 59.795 | 58.793 | End: 363 |
| Size | 21 | 26 | 125 |
| Primer 2 details |
|---|
| Sequence | TCAGCCGATTGTGGTCTACTG | TCGCACTCCAAATTTAAGAAAATTGT | Start: 238 |
| Tm | 59.795 | 58.793 | End: 363 |
| Size | 21 | 26 | 126 |
| Primer 3 details |
|---|
| Sequence | CAGCCGATTGTGGTCTACTGA | CGCACTCCAAATTTAAGAAAATTGT | Start: 239 |
| Tm | 59.795 | 57.593 | End: 362 |
| Size | 21 | 25 | 124 |
| Primer 4 details |
|---|
| Sequence | TCAGCCGATTGTGGTCTACTG | CGCACTCCAAATTTAAGAAAATTGT | Start: 238 |
| Tm | 59.795 | 57.593 | End: 362 |
| Size | 21 | 25 | 125 |
| Primer 5 details |
|---|
| Sequence | AGCCGATTGTGGTCTACTGA | TCGCACTCCAAATTTAAGAAAATTGT | Start: 240 |
| Tm | 58.443 | 58.793 | End: 363 |
| Size | 20 | 26 | 124 |
(T)10atatttgtaaatatattcatctttc(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGCCGATTGTGGTCTACTGA | TCGCACTCCAAATTTAAGAAAATTGT | Start: 239 |
| Tm | 59.795 | 58.793 | End: 363 |
| Size | 21 | 26 | 125 |
| Primer 2 details |
|---|
| Sequence | TCAGCCGATTGTGGTCTACTG | TCGCACTCCAAATTTAAGAAAATTGT | Start: 238 |
| Tm | 59.795 | 58.793 | End: 363 |
| Size | 21 | 26 | 126 |
| Primer 3 details |
|---|
| Sequence | CAGCCGATTGTGGTCTACTGA | CGCACTCCAAATTTAAGAAAATTGT | Start: 239 |
| Tm | 59.795 | 57.593 | End: 362 |
| Size | 21 | 25 | 124 |
| Primer 4 details |
|---|
| Sequence | TCAGCCGATTGTGGTCTACTG | CGCACTCCAAATTTAAGAAAATTGT | Start: 238 |
| Tm | 59.795 | 57.593 | End: 362 |
| Size | 21 | 25 | 125 |
| Primer 5 details |
|---|
| Sequence | AGCCGATTGTGGTCTACTGA | TCGCACTCCAAATTTAAGAAAATTGT | Start: 240 |
| Tm | 58.443 | 58.793 | End: 363 |
| Size | 20 | 26 | 124 |
