ssr_primer
Primer Details for TRINITY_DN5449_c0_g1_i1 |
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| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAATGTGGCCGGTGAAAGGG | ACACGGTAAATGGTCCAACTGA | Start: 42 |
| Tm | 60.323 | 59.895 | End: 148 |
| Size | 20 | 22 | 107 |
| Primer 2 details |
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| Sequence | TCCTAATGTGGCCGGTGAAA | ACACGGTAAATGGTCCAACTGA | Start: 39 |
| Tm | 59.306 | 59.895 | End: 148 |
| Size | 20 | 22 | 110 |
| Primer 3 details |
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| Sequence | TAATGTGGCCGGTGAAAGGG | ACACGGTAAATGGTCCAACTG | Start: 42 |
| Tm | 60.323 | 58.5 | End: 148 |
| Size | 20 | 21 | 107 |
| Primer 4 details |
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| Sequence | CCGGTGAAAGGGTCATCCAT | ACAACACGGTAAATGGTCCAAC | Start: 50 |
| Tm | 59.744 | 59.38 | End: 151 |
| Size | 20 | 22 | 102 |
| Primer 5 details |
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| Sequence | CCGGTGAAAGGGTCATCCAT | ACAACACGGTAAATGGTCCA | Start: 50 |
| Tm | 59.744 | 57.358 | End: 151 |
| Size | 20 | 20 | 102 |
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