ssr_primer
Primer Details for TRINITY_DN5364_c0_g1_i1 |
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| (AAG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGACTAGCAGATGGGTTGT | TTGTCAAGCCTTGCAAGAGC | Start: 349 |
| Tm | 58.739 | 59.33 | End: 505 |
| Size | 21 | 20 | 157 |
| Primer 2 details |
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| Sequence | AGTGACTAGCAGATGGGTTGT | TTTGTCAAGCCTTGCAAGAGC | Start: 349 |
| Tm | 58.739 | 59.932 | End: 506 |
| Size | 21 | 21 | 158 |
| Primer 3 details |
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| Sequence | GTGACTAGCAGATGGGTTGT | TTGTCAAGCCTTGCAAGAGC | Start: 350 |
| Tm | 57.23 | 59.33 | End: 505 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | AGTGACTAGCAGATGGGTTGT | ACGATGCAACTTCCACTCTGA | Start: 349 |
| Tm | 58.739 | 59.656 | End: 473 |
| Size | 21 | 21 | 125 |
| Primer 5 details |
|---|
| Sequence | TGTAGTGACTAGCAGATGGGT | TTGTCAAGCCTTGCAAGAGC | Start: 346 |
| Tm | 57.898 | 59.33 | End: 505 |
| Size | 21 | 20 | 160 |
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