Primer Details for TRINITY_DN5176_c0_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCATGGATTCATCAAGGTTG | TGGATTTCTTGCATTTTTCAACATGG | Start: 60 |
| Tm | 59.238 | 59.795 | End: 215 |
| Size | 22 | 26 | 156 |
| Primer 2 details |
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| Sequence | TGGCATGGATTCATCAAGGT | TGGATTTCTTGCATTTTTCAACATGG | Start: 60 |
| Tm | 57.167 | 59.795 | End: 215 |
| Size | 20 | 26 | 156 |
| Primer 3 details |
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| Sequence | TGGCATGGATTCATCAAGGTTG | AGTGGATTTCTTGCATTTTTCAACA | Start: 60 |
| Tm | 59.238 | 58.652 | End: 217 |
| Size | 22 | 25 | 158 |
| Primer 4 details |
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| Sequence | TGGCATGGATTCATCAAGGTTG | AGAGTGGATTTCTTGCATTTTTCA | Start: 60 |
| Tm | 59.238 | 57.59 | End: 219 |
| Size | 22 | 24 | 160 |
| Primer 5 details |
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| Sequence | TGGCATGGATTCATCAAGGT | AGTGGATTTCTTGCATTTTTCAACA | Start: 60 |
| Tm | 57.167 | 58.652 | End: 217 |
| Size | 20 | 25 | 158 |
