Primer Details for TRINITY_DN5081_c1_g1_i1 |
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| (A)26: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAACAGCTAGGAGGGAAGA | GCTAATATAGTGTAGCTATAGACCCT | Start: 99 |
| Tm | 59.921 | 57.016 | End: 265 |
| Size | 21 | 26 | 167 |
| Primer 2 details |
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| Sequence | AGCCCAAGTTGTCACTTCGC | GCTAATATAGTGTAGCTATAGACCCT | Start: 127 |
| Tm | 61.167 | 57.016 | End: 265 |
| Size | 20 | 26 | 139 |
| Primer 3 details |
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| Sequence | CCCAAGTTGTCACTTCGCAT | GCTAATATAGTGTAGCTATAGACCCT | Start: 129 |
| Tm | 58.762 | 57.016 | End: 265 |
| Size | 20 | 26 | 137 |
| Primer 4 details |
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| Sequence | GTTGGAACAGCTAGGAGGGA | GCTAATATAGTGTAGCTATAGACCCT | Start: 97 |
| Tm | 58.723 | 57.016 | End: 265 |
| Size | 20 | 26 | 169 |
| Primer 5 details |
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| Sequence | GCCCAAGTTGTCACTTCGC | GCTAATATAGTGTAGCTATAGACCCT | Start: 128 |
| Tm | 59.717 | 57.016 | End: 265 |
| Size | 19 | 26 | 138 |
