Primer Details for TRINITY_DN5071_c0_g1_i1 |
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| (TTA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGATCGAAAAAGATAAAAGAATCGGA | TCCCTCTCGTTTCATGTCATT | Start: 70 |
| Tm | 57.513 | 57.019 | End: 198 |
| Size | 26 | 21 | 129 |
| Primer 2 details |
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| Sequence | TGATCGAAAAAGATAAAAGAATCGGA | AATCCCTCTCGTTTCATGTCA | Start: 70 |
| Tm | 57.513 | 57.019 | End: 200 |
| Size | 26 | 21 | 131 |
| Primer 3 details |
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| Sequence | TGATCGAAAAAGATAAAAGAATCGGA | TCCCTCTCGTTTCATGTCATTT | Start: 70 |
| Tm | 57.513 | 57.714 | End: 198 |
| Size | 26 | 22 | 129 |
| Primer 4 details |
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| Sequence | TCGAAAAAGATAAAAGAATCGGAATGA | TCCCTCTCGTTTCATGTCATT | Start: 73 |
| Tm | 58.058 | 57.019 | End: 198 |
| Size | 27 | 21 | 126 |
| Primer 5 details |
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| Sequence | TCGAAAAAGATAAAAGAATCGGAATGA | AATCCCTCTCGTTTCATGTCA | Start: 73 |
| Tm | 58.058 | 57.019 | End: 200 |
| Size | 27 | 21 | 128 |
