Primer Details for TRINITY_DN5054_c0_g1_i1 |
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| (AG)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGACCAAACAGACTGGAA | TGCTACATGAAAAATGGTGAACCT | Start: 99 |
| Tm | 60.534 | 59.168 | End: 323 |
| Size | 20 | 24 | 225 |
| Primer 2 details |
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| Sequence | AAGCCGACCAAACAGACTGG | TGCTACATGAAAAATGGTGAACCT | Start: 97 |
| Tm | 60.535 | 59.168 | End: 323 |
| Size | 20 | 24 | 227 |
| Primer 3 details |
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| Sequence | GCCGACCAAACAGACTGGAA | GCTACATGAAAAATGGTGAACCTCA | Start: 99 |
| Tm | 60.534 | 60.048 | End: 322 |
| Size | 20 | 25 | 224 |
| Primer 4 details |
|---|
| Sequence | GCCGACCAAACAGACTGGAA | TGCTACATGAAAAATGGTGAACCTC | Start: 99 |
| Tm | 60.534 | 60.048 | End: 323 |
| Size | 20 | 25 | 225 |
| Primer 5 details |
|---|
| Sequence | AAGCCGACCAAACAGACTGG | GCTACATGAAAAATGGTGAACCTCA | Start: 97 |
| Tm | 60.535 | 60.048 | End: 322 |
| Size | 20 | 25 | 226 |
