Primer Details for TRINITY_DN5022_c0_g1_i1 |
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| (T)10ctaaaatgagaccctacatcgcaaaatataacgtgcatttccatttttccattatt(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCAAACACAAACATTAGAGAGTG | TGGAAAAATGGAAATGCACGT | Start: 147 |
| Tm | 59.701 | 57.258 | End: 246 |
| Size | 25 | 21 | 100 |
| Primer 2 details |
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| Sequence | ACCCAAACACAAACATTAGAGAGT | TGGAAAAATGGAAATGCACGT | Start: 147 |
| Tm | 58.554 | 57.258 | End: 246 |
| Size | 24 | 21 | 100 |
| Primer 3 details |
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| Sequence | ACCCAAACACAAACATTAGAGAGTG | TGGAAAAATGGAAATGCACGTT | Start: 147 |
| Tm | 59.701 | 57.935 | End: 246 |
| Size | 25 | 22 | 100 |
| Primer 4 details |
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| Sequence | ACCCAAACACAAACATTAGAGAGT | TGGAAAAATGGAAATGCACGTT | Start: 147 |
| Tm | 58.554 | 57.935 | End: 246 |
| Size | 24 | 22 | 100 |
| Primer 5 details |
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| Sequence | CCCAAACACAAACATTAGAGAGTGG | ATGGAAAAATGGAAATGCACGT | Start: 148 |
| Tm | 60.278 | 57.73 | End: 247 |
| Size | 25 | 22 | 100 |
