ssr_primer
Primer Details for TRINITY_DN499_c0_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACCTTCTTCCCGATGCAA | CCAACTGCCTCGTCTTCACT | Start: 443 |
| Tm | 60.035 | 59.966 | End: 595 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | GGACCTTCTTCCCGATGCAA | GCAAACTCTCCTCCGGAACA | Start: 443 |
| Tm | 60.035 | 59.965 | End: 574 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | AGTGAAGACGAGGCAGTTGG | CGGTCAGGATCCGTTGGAAA | Start: 576 |
| Tm | 59.966 | 60.037 | End: 850 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
|---|
| Sequence | GGACCTTCTTCCCGATGCAA | AACTGCCTCGTCTTCACTCG | Start: 443 |
| Tm | 60.035 | 60.039 | End: 593 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | CGAGTGAAGACGAGGCAGTT | CGGTCAGGATCCGTTGGAAA | Start: 574 |
| Tm | 60.039 | 60.037 | End: 850 |
| Size | 20 | 20 | 277 |
(GCG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACCTTCTTCCCGATGCAA | CCAACTGCCTCGTCTTCACT | Start: 443 |
| Tm | 60.035 | 59.966 | End: 595 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | GGACCTTCTTCCCGATGCAA | GCAAACTCTCCTCCGGAACA | Start: 443 |
| Tm | 60.035 | 59.965 | End: 574 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | AGTGAAGACGAGGCAGTTGG | CGGTCAGGATCCGTTGGAAA | Start: 576 |
| Tm | 59.966 | 60.037 | End: 850 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
|---|
| Sequence | GGACCTTCTTCCCGATGCAA | AACTGCCTCGTCTTCACTCG | Start: 443 |
| Tm | 60.035 | 60.039 | End: 593 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | CGAGTGAAGACGAGGCAGTT | CGGTCAGGATCCGTTGGAAA | Start: 574 |
| Tm | 60.039 | 60.037 | End: 850 |
| Size | 20 | 20 | 277 |
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