ssr_primer
Primer Details for TRINITY_DN4888_c0_g1_i1 |
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| (TTTTC)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGCTTTGGTTGTTCGACAC | CGGCATAGTACACACAACGG | Start: 217 |
| Tm | 59.97 | 59.004 | End: 458 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
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| Sequence | TGTGTCTAGCTAGCTCCTGGT | GTGTCGAACAACCAAAGCCC | Start: 136 |
| Tm | 59.994 | 59.97 | End: 236 |
| Size | 21 | 20 | 101 |
| Primer 3 details |
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| Sequence | TGTTCCGTATGTTGCCGAGG | AAAAAGAAACAGAGGCCGGC | Start: 198 |
| Tm | 60.391 | 59.326 | End: 311 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
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| Sequence | GCTGTGTCTAGCTAGCTCCTG | GTGTCGAACAACCAAAGCCC | Start: 134 |
| Tm | 59.933 | 59.97 | End: 236 |
| Size | 21 | 20 | 103 |
| Primer 5 details |
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| Sequence | CTGCTGTGTCTAGCTAGCTCC | GTGTCGAACAACCAAAGCCC | Start: 132 |
| Tm | 59.933 | 59.97 | End: 236 |
| Size | 21 | 20 | 105 |
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