ssr_primer
Primer Details for TRINITY_DN478_c0_g1_i1 |
|---|
| (AT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGATGCCTTAACTTTGGAGC | TGGCTGATTTTGACAGGGACA | Start: 97 |
| Tm | 58.915 | 59.855 | End: 347 |
| Size | 22 | 21 | 251 |
| Primer 2 details |
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| Sequence | ACTGATGCCTTAACTTTGGAGCT | TGGCTGATTTTGACAGGGACA | Start: 97 |
| Tm | 60.245 | 59.855 | End: 347 |
| Size | 23 | 21 | 251 |
| Primer 3 details |
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| Sequence | TCTTATGTTTGCTCCCTTTCCCT | TGGCTGATTTTGACAGGGACA | Start: 122 |
| Tm | 59.666 | 59.855 | End: 347 |
| Size | 23 | 21 | 226 |
| Primer 4 details |
|---|
| Sequence | CTGATGCCTTAACTTTGGAGCT | TGGCTGATTTTGACAGGGACA | Start: 98 |
| Tm | 58.649 | 59.855 | End: 347 |
| Size | 22 | 21 | 250 |
| Primer 5 details |
|---|
| Sequence | TGATGCCTTAACTTTGGAGCT | TGGCTGATTTTGACAGGGACA | Start: 99 |
| Tm | 57.56 | 59.855 | End: 347 |
| Size | 21 | 21 | 249 |
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