ssr_primer
Primer Details for TRINITY_DN4657_c0_g1_i1 |
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| (G)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGAAAGTGGGGTCAAGG | CCCCCTCCTTTTTACTCCTTCC | Start: 201 |
| Tm | 59.591 | 60.025 | End: 302 |
| Size | 20 | 22 | 102 |
| Primer 2 details |
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| Sequence | GGGAAAGTGGGGTCAAGGG | CCCCCTCCTTTTTACTCCTTCC | Start: 203 |
| Tm | 59.923 | 60.025 | End: 302 |
| Size | 19 | 22 | 100 |
| Primer 3 details |
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| Sequence | AATGGGAAAGTGGGGTCAAGG | CCCCCTCCTTTTTACTCCTTCC | Start: 200 |
| Tm | 60.203 | 60.025 | End: 302 |
| Size | 21 | 22 | 103 |
| Primer 4 details |
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| Sequence | ATGGGAAAGTGGGGTCAAGG | CCCCTCCTTTTTACTCCTTCCT | Start: 201 |
| Tm | 59.591 | 59.08 | End: 301 |
| Size | 20 | 22 | 101 |
| Primer 5 details |
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| Sequence | GGGAAAGTGGGGTCAAGGGA | CCCCCTCCTTTTTACTCCTTCC | Start: 203 |
| Tm | 61.432 | 60.025 | End: 302 |
| Size | 20 | 22 | 100 |
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