Primer Details for TRINITY_DN4656_c0_g1_i1 |
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| (GAAAA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCATAGGAGTAATTATTTACTTGGGG | ACAACACCAATTACTCACAAGATGA | Start: 136 |
| Tm | 58.554 | 58.939 | End: 245 |
| Size | 27 | 25 | 110 |
| Primer 2 details |
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| Sequence | ACCATAGGAGTAATTATTTACTTGGGG | GGTAACAACACCAATTACTCACAAGA | Start: 136 |
| Tm | 58.554 | 59.739 | End: 249 |
| Size | 27 | 26 | 114 |
| Primer 3 details |
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| Sequence | CCATAGGAGTAATTATTTACTTGGGGA | ACAACACCAATTACTCACAAGATGA | Start: 137 |
| Tm | 58.334 | 58.939 | End: 245 |
| Size | 27 | 25 | 109 |
| Primer 4 details |
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| Sequence | ACCATAGGAGTAATTATTTACTTGGGG | ACACCAATTACTCACAAGATGAACT | Start: 136 |
| Tm | 58.554 | 58.701 | End: 242 |
| Size | 27 | 25 | 107 |
| Primer 5 details |
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| Sequence | ACCATAGGAGTAATTATTTACTTGGGG | ACAACACCAATTACTCACAAGATG | Start: 136 |
| Tm | 58.554 | 57.682 | End: 245 |
| Size | 27 | 24 | 110 |
