Primer Details for TRINITY_DN4572_c0_g1_i1 | |||
|---|---|---|---|
| (T)40: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTTAGAAACCAAACTCTCTGT | TCTCCTCATCACTTGATCCGAC | Start: 190 |
| Tm | 57.728 | 59.31 | End: 351 |
| Size | 23 | 22 | 162 |
| Primer 2 details | |||
| Sequence | TCGTTAGAAACCAAACTCTCTGT | CTCCTCATCACTTGATCCGACT | Start: 190 |
| Tm | 57.728 | 59.307 | End: 350 |
| Size | 23 | 22 | 161 |
| Primer 3 details | |||
| Sequence | TCGTTAGAAACCAAACTCTCTGT | TCCTCATCACTTGATCCGACT | Start: 190 |
| Tm | 57.728 | 58.26 | End: 349 |
| Size | 23 | 21 | 160 |
| Primer 4 details | |||
| Sequence | TGCTAACCTATTTCCTACTGAACGA | TCTCCTCATCACTTGATCCGAC | Start: 98 |
| Tm | 59.58 | 59.31 | End: 351 |
| Size | 25 | 22 | 254 |
| Primer 5 details | |||
| Sequence | TGCTAACCTATTTCCTACTGAACGA | CTCCTCATCACTTGATCCGACT | Start: 98 |
| Tm | 59.58 | 59.307 | End: 350 |
| Size | 25 | 22 | 253 |
