Primer Details for TRINITY_DN4518_c0_g1_i1 |
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| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAACATTCCACTTTCCCCC | TGATCTTGTGTGTCATTCTTTTGA | Start: 55 |
| Tm | 59.392 | 57.059 | End: 174 |
| Size | 20 | 24 | 120 |
| Primer 2 details |
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| Sequence | GGCAACATTCCACTTTCCCC | TGATCTTGTGTGTCATTCTTTTGA | Start: 54 |
| Tm | 59.392 | 57.059 | End: 174 |
| Size | 20 | 24 | 121 |
| Primer 3 details |
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| Sequence | GCAACATTCCACTTTCCCCC | TGTGTGTCATTCTTTTGATTGATCAGA | Start: 55 |
| Tm | 59.392 | 59.771 | End: 168 |
| Size | 20 | 27 | 114 |
| Primer 4 details |
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| Sequence | GGCAACATTCCACTTTCCCC | TGTGTGTCATTCTTTTGATTGATCAGA | Start: 54 |
| Tm | 59.392 | 59.771 | End: 168 |
| Size | 20 | 27 | 115 |
| Primer 5 details |
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| Sequence | GCAACATTCCACTTTCCCCC | TGTGTGTCATTCTTTTGATTGATCA | Start: 55 |
| Tm | 59.392 | 57.725 | End: 168 |
| Size | 20 | 25 | 114 |
