Primer Details for TRINITY_DN4414_c0_g1_i1 | |||
|---|---|---|---|
| (AAT)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGACGGCTATCATACGGT | ACCAACTTTAGCTCACTAACTTGA | Start: 127 |
| Tm | 58.023 | 58.074 | End: 361 |
| Size | 20 | 24 | 235 |
| Primer 2 details | |||
| Sequence | TCTGACGGCTATCATACGGTT | ACCAACTTTAGCTCACTAACTTGA | Start: 127 |
| Tm | 58.696 | 58.074 | End: 361 |
| Size | 21 | 24 | 235 |
| Primer 3 details | |||
| Sequence | TCTGACGGCTATCATACGGT | ACCAACTTTAGCTCACTAACTTGAA | Start: 127 |
| Tm | 58.023 | 58.646 | End: 361 |
| Size | 20 | 25 | 235 |
| Primer 4 details | |||
| Sequence | TCTGACGGCTATCATACGGT | AACCAACTTTAGCTCACTAACTTGA | Start: 127 |
| Tm | 58.023 | 58.646 | End: 362 |
| Size | 20 | 25 | 236 |
| Primer 5 details | |||
| Sequence | ATCTGACGGCTATCATACGGT | ACCAACTTTAGCTCACTAACTTGA | Start: 126 |
| Tm | 58.484 | 58.074 | End: 361 |
| Size | 21 | 24 | 236 |
