Primer Details for TRINITY_DN4171_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAACAGTTATCGCTTTACAAAGG | TGCTAGAGGGATATGGATGAGA | Start: 69 |
| Tm | 59.82 | 57.318 | End: 171 |
| Size | 25 | 22 | 103 |
| Primer 2 details |
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| Sequence | GGAACAGTTATCGCTTTACAAAGGA | TGCTAGAGGGATATGGATGAGA | Start: 70 |
| Tm | 59.59 | 57.318 | End: 171 |
| Size | 25 | 22 | 102 |
| Primer 3 details |
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| Sequence | GGAACAGTTATCGCTTTACAAAGG | TGCTAGAGGGATATGGATGAGA | Start: 70 |
| Tm | 58.372 | 57.318 | End: 171 |
| Size | 24 | 22 | 102 |
| Primer 4 details |
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| Sequence | TGGAACAGTTATCGCTTTACAAAGG | TGCTAGAGGGATATGGATGAGAT | Start: 69 |
| Tm | 59.82 | 57.782 | End: 171 |
| Size | 25 | 23 | 103 |
| Primer 5 details |
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| Sequence | TGGAACAGTTATCGCTTTACAAAGG | ATGCTAGAGGGATATGGATGAGA | Start: 69 |
| Tm | 59.82 | 57.782 | End: 172 |
| Size | 25 | 23 | 104 |
