Primer Details for TRINITY_DN40717_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTGTCATGTAAAGATTCTGGAGC | AAGGAAGGGCAAATTGGTGA | Start: 8 |
| Tm | 59.123 | 57.603 | End: 107 |
| Size | 25 | 20 | 100 |
| Primer 2 details |
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| Sequence | CTTGTCATGTAAAGATTCTGGAGCA | AAAGGAAGGGCAAATTGGTGA | Start: 9 |
| Tm | 59.355 | 58.312 | End: 108 |
| Size | 25 | 21 | 100 |
| Primer 3 details |
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| Sequence | TCTTGTCATGTAAAGATTCTGGAGC | AAGGAAGGGCAAATTGGTGAA | Start: 8 |
| Tm | 59.123 | 58.312 | End: 107 |
| Size | 25 | 21 | 100 |
| Primer 4 details |
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| Sequence | TCTTGTCATGTAAAGATTCTGGAGC | AAAGGAAGGGCAAATTGGTGA | Start: 8 |
| Tm | 59.123 | 58.312 | End: 108 |
| Size | 25 | 21 | 101 |
| Primer 5 details |
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| Sequence | GTCTTGTCATGTAAAGATTCTGGAGC | AGGAAGGGCAAATTGGTGAA | Start: 7 |
| Tm | 60.183 | 57.603 | End: 106 |
| Size | 26 | 20 | 100 |
