Primer Details for TRINITY_DN40645_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGGACTCAAGTTTAATTCAACTTTT | TCTGGGTCTGAGGTCTTTGGA | Start: 3 |
| Tm | 57.063 | 60.133 | End: 169 |
| Size | 27 | 21 | 167 |
| Primer 2 details |
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| Sequence | TCACTTAAAAGACTACACTAAGTGCC | AGAAGAAAAATCTGGGTCTGAGGT | Start: 79 |
| Tm | 59.073 | 59.648 | End: 179 |
| Size | 26 | 24 | 101 |
| Primer 3 details |
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| Sequence | CACTTAAAAGACTACACTAAGTGCCT | AGAAGAAAAATCTGGGTCTGAGGT | Start: 80 |
| Tm | 59.07 | 59.648 | End: 179 |
| Size | 26 | 24 | 100 |
| Primer 4 details |
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| Sequence | TTTGGACTCAAGTTTAATTCAACTTTT | CTGGGTCTGAGGTCTTTGGA | Start: 3 |
| Tm | 57.063 | 58.648 | End: 168 |
| Size | 27 | 20 | 166 |
| Primer 5 details |
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| Sequence | TTTGGACTCAAGTTTAATTCAACTTTT | TCTGGGTCTGAGGTCTTTGG | Start: 3 |
| Tm | 57.063 | 58.648 | End: 169 |
| Size | 27 | 20 | 167 |
