Primer Details for TRINITY_DN40614_c0_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAGTGTAAGAGTTCATAGTTGAGT | GCAAGGGCATTCTCCAGAGA | Start: 42 |
| Tm | 59.278 | 59.746 | End: 203 |
| Size | 26 | 20 | 162 |
| Primer 2 details |
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| Sequence | GGAGTGTAAGAGTTCATAGTTGAGT | GCAAGGGCATTCTCCAGAGA | Start: 43 |
| Tm | 58.074 | 59.746 | End: 203 |
| Size | 25 | 20 | 161 |
| Primer 3 details |
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| Sequence | AGGAGTGTAAGAGTTCATAGTTGAG | GCAAGGGCATTCTCCAGAGA | Start: 42 |
| Tm | 57.836 | 59.746 | End: 203 |
| Size | 25 | 20 | 162 |
| Primer 4 details |
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| Sequence | AGGAGTGTAAGAGTTCATAGTTGAGTT | GCAAGGGCATTCTCCAGAGA | Start: 42 |
| Tm | 59.766 | 59.746 | End: 203 |
| Size | 27 | 20 | 162 |
| Primer 5 details |
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| Sequence | AAGGAGTGTAAGAGTTCATAGTTGAGT | GCAAGGGCATTCTCCAGAGA | Start: 41 |
| Tm | 59.766 | 59.746 | End: 203 |
| Size | 27 | 20 | 163 |
