Primer Details for TRINITY_DN40544_c0_g1_i1 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAACACAACACATGATTAACCAA | GCCCGCTATCCTCTCCCTTA | Start: 174 |
| Tm | 57.293 | 60.542 | End: 363 |
| Size | 24 | 20 | 190 |
| Primer 2 details | |||
| Sequence | AGAACACAACACATGATTAACCAAA | GCCCGCTATCCTCTCCCTTA | Start: 174 |
| Tm | 57.894 | 60.542 | End: 363 |
| Size | 25 | 20 | 190 |
| Primer 3 details | |||
| Sequence | AGAACACAACACATGATTAACCAA | CCCGCTATCCTCTCCCTTACT | Start: 174 |
| Tm | 57.293 | 60.203 | End: 362 |
| Size | 24 | 21 | 189 |
| Primer 4 details | |||
| Sequence | AGAACACAACACATGATTAACCAA | CCCGCTATCCTCTCCCTTAC | Start: 174 |
| Tm | 57.293 | 58.741 | End: 362 |
| Size | 24 | 20 | 189 |
| Primer 5 details | |||
| Sequence | AGAACACAACACATGATTAACCAA | GCTTGCCCGCTATCCTCTC | Start: 174 |
| Tm | 57.293 | 60.301 | End: 367 |
| Size | 24 | 19 | 194 |
