Primer Details for TRINITY_DN40500_c0_g1_i1 |
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| (TA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTAATGCAAAACTTCAAACTTTCA | TGTGTTATGTGAGTTTCAAGGTTCTG | Start: 26 |
| Tm | 58.316 | 59.962 | End: 152 |
| Size | 26 | 26 | 127 |
| Primer 2 details |
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| Sequence | GCTTAATGCAAAACTTCAAACTTTCA | GTGTTATGTGAGTTTCAAGGTTCTGT | Start: 26 |
| Tm | 58.316 | 59.962 | End: 151 |
| Size | 26 | 26 | 126 |
| Primer 3 details |
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| Sequence | GCTTAATGCAAAACTTCAAACTTTCA | TGTTATGTGAGTTTCAAGGTTCTGT | Start: 26 |
| Tm | 58.316 | 58.88 | End: 150 |
| Size | 26 | 25 | 125 |
| Primer 4 details |
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| Sequence | GCTTAATGCAAAACTTCAAACTTTCA | TGTGTTATGTGAGTTTCAAGGTTCT | Start: 26 |
| Tm | 58.316 | 58.88 | End: 152 |
| Size | 26 | 25 | 127 |
| Primer 5 details |
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| Sequence | GCTTAATGCAAAACTTCAAACTTTCA | TGTGTTATGTGAGTTTCAAGGTTC | Start: 26 |
| Tm | 58.316 | 57.628 | End: 152 |
| Size | 26 | 24 | 127 |
