Primer Details for TRINITY_DN40458_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCAATAAAACGAATCCGCG | TTACGGTCAACGGAAACTTTTT | Start: 101 |
| Tm | 59.937 | 57.039 | End: 305 |
| Size | 21 | 22 | 205 |
| Primer 2 details | |||
| Sequence | GCCAATAAAACGAATCCGCG | TTACGGTCAACGGAAACTTTTT | Start: 102 |
| Tm | 58.537 | 57.039 | End: 305 |
| Size | 20 | 22 | 204 |
| Primer 3 details | |||
| Sequence | ACGACCAGCCAATAAAACGA | TTACGGTCAACGGAAACTTTTT | Start: 95 |
| Tm | 57.827 | 57.039 | End: 305 |
| Size | 20 | 22 | 211 |
| Primer 4 details | |||
| Sequence | AGCCAATAAAACGAATCCGCG | GCACCGTGTCAAATATAATAGGATCC | Start: 101 |
| Tm | 59.937 | 59.908 | End: 240 |
| Size | 21 | 26 | 140 |
| Primer 5 details | |||
| Sequence | AGCCAATAAAACGAATCCGCG | AGCACCGTGTCAAATATAATAGGA | Start: 101 |
| Tm | 59.937 | 57.83 | End: 241 |
| Size | 21 | 24 | 141 |
