Primer Details for TRINITY_DN40431_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGATAATAGAGAAGAACACACGA | TGGGCATGTTGATGGGTCTC | Start: 46 |
| Tm | 59.345 | 60.034 | End: 165 |
| Size | 25 | 20 | 120 |
| Primer 2 details |
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| Sequence | GTGGGATAATAGAGAAGAACACACG | TGGGCATGTTGATGGGTCTC | Start: 45 |
| Tm | 59.251 | 60.034 | End: 165 |
| Size | 25 | 20 | 121 |
| Primer 3 details |
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| Sequence | TGGGATAATAGAGAAGAACACACGA | TTGTGGGCATGTTGATGGGT | Start: 46 |
| Tm | 59.345 | 60.179 | End: 168 |
| Size | 25 | 20 | 123 |
| Primer 4 details |
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| Sequence | TGGGATAATAGAGAAGAACACACGA | GGGCATGTTGATGGGTCTCT | Start: 46 |
| Tm | 59.345 | 59.743 | End: 164 |
| Size | 25 | 20 | 119 |
| Primer 5 details |
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| Sequence | GTGGGATAATAGAGAAGAACACACG | TTGTGGGCATGTTGATGGGT | Start: 45 |
| Tm | 59.251 | 60.179 | End: 168 |
| Size | 25 | 20 | 124 |
