Primer Details for TRINITY_DN40375_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTTGTCAAGCAAGGACTCA | TGATCAAAATGATTGAGGTTTCATGA | Start: 71 |
| Tm | 59.788 | 57.875 | End: 195 |
| Size | 21 | 26 | 125 |
| Primer 2 details |
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| Sequence | TGGTTGTCAAGCAAGGACTCA | ACATTGATCAAAATGATTGAGGTTTCA | Start: 71 |
| Tm | 59.788 | 58.631 | End: 199 |
| Size | 21 | 27 | 129 |
| Primer 3 details |
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| Sequence | TGGTTGTCAAGCAAGGACTCA | TTGATCAAAATGATTGAGGTTTCATGA | Start: 71 |
| Tm | 59.788 | 58.413 | End: 196 |
| Size | 21 | 27 | 126 |
| Primer 4 details |
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| Sequence | TGGTTGTCAAGCAAGGACTCA | TGATCAAAATGATTGAGGTTTCATGAT | Start: 71 |
| Tm | 59.788 | 58.245 | End: 195 |
| Size | 21 | 27 | 125 |
| Primer 5 details |
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| Sequence | TGGTTGTCAAGCAAGGACTCA | TTGATCAAAATGATTGAGGTTTCATG | Start: 71 |
| Tm | 59.788 | 57.234 | End: 196 |
| Size | 21 | 26 | 126 |
