Primer Details for TRINITY_DN40251_c0_g1_i1 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTTCTGTTTGAACTATCTGA | TCAAGGTCTGACTATTCGAAAACA | Start: 18 |
| Tm | 57.772 | 58.15 | End: 194 |
| Size | 23 | 24 | 177 |
| Primer 2 details | |||
| Sequence | TGCCTTCTGTTTGAACTATCTGA | TTCAAGGTCTGACTATTCGAAAACA | Start: 18 |
| Tm | 57.772 | 58.715 | End: 195 |
| Size | 23 | 25 | 178 |
| Primer 3 details | |||
| Sequence | TGCCTTCTGTTTGAACTATCTGA | AGGTCTGACTATTCGAAAACATCT | Start: 18 |
| Tm | 57.772 | 57.712 | End: 191 |
| Size | 23 | 24 | 174 |
| Primer 4 details | |||
| Sequence | TGCCTTCTGTTTGAACTATCTGAT | TCAAGGTCTGACTATTCGAAAACA | Start: 18 |
| Tm | 58.191 | 58.15 | End: 194 |
| Size | 24 | 24 | 177 |
| Primer 5 details | |||
| Sequence | ATGCCTTCTGTTTGAACTATCTGA | TCAAGGTCTGACTATTCGAAAACA | Start: 17 |
| Tm | 58.191 | 58.15 | End: 194 |
| Size | 24 | 24 | 178 |
