Primer Details for TRINITY_DN40092_c0_g1_i1 | |||
|---|---|---|---|
| (AAG)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGATTCCACACCTGCGCTC | AGCGCACTTCTCATATAGTTTCT | Start: 192 |
| Tm | 59.824 | 57.73 | End: 306 |
| Size | 20 | 23 | 115 |
| Primer 2 details | |||
| Sequence | TGCGCTCTCACTGTTGTGTA | AGCGCACTTCTCATATAGTTTCT | Start: 205 |
| Tm | 59.612 | 57.73 | End: 306 |
| Size | 20 | 23 | 102 |
| Primer 3 details | |||
| Sequence | TAGATTCCACACCTGCGCTC | AGCGCACTTCTCATATAGTTTCTT | Start: 192 |
| Tm | 59.824 | 58.335 | End: 306 |
| Size | 20 | 24 | 115 |
| Primer 4 details | |||
| Sequence | TAGATTCCACACCTGCGCTC | AAGCGCACTTCTCATATAGTTTCT | Start: 192 |
| Tm | 59.824 | 58.335 | End: 307 |
| Size | 20 | 24 | 116 |
| Primer 5 details | |||
| Sequence | ATAGATTCCACACCTGCGCT | AGTTTCTTACCTTGTCACTGATGGA | Start: 191 |
| Tm | 59.167 | 59.928 | End: 290 |
| Size | 20 | 25 | 100 |
