Primer Details for TRINITY_DN3985_c0_g1_i1 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTCTTTCCGTATATGTAGTTTGTGA | GTATGTAAACTTTGCATACGCGT | Start: 24 |
| Tm | 57.508 | 58.111 | End: 209 |
| Size | 27 | 23 | 186 |
| Primer 2 details |
|---|
| Sequence | TCCGTATATGTAGTTTGTGATTTTCTT | GTATGTAAACTTTGCATACGCGT | Start: 31 |
| Tm | 57.338 | 58.111 | End: 209 |
| Size | 27 | 23 | 179 |
| Primer 3 details |
|---|
| Sequence | TTCCGTATATGTAGTTTGTGATTTTCT | GTATGTAAACTTTGCATACGCGT | Start: 30 |
| Tm | 57.338 | 58.111 | End: 209 |
| Size | 27 | 23 | 180 |
| Primer 4 details |
|---|
| Sequence | TCTTTCCGTATATGTAGTTTGTGATTT | GTATGTAAACTTTGCATACGCGT | Start: 27 |
| Tm | 57.338 | 58.111 | End: 209 |
| Size | 27 | 23 | 183 |
| Primer 5 details |
|---|
| Sequence | TTCTTTCCGTATATGTAGTTTGTGATT | GTATGTAAACTTTGCATACGCGT | Start: 26 |
| Tm | 57.338 | 58.111 | End: 209 |
| Size | 27 | 23 | 184 |
