Primer Details for TRINITY_DN39832_c0_g1_i1 | |||
|---|---|---|---|
| (TTA)5gttg(TTA)6: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTAGAAGCAAATCATACAAGGA | GATGACTCTGTCGTTCGACG | Start: 115 |
| Tm | 57.939 | 58.474 | End: 269 |
| Size | 24 | 20 | 155 |
| Primer 2 details | |||
| Sequence | TGGTAGAAGCAAATCATACAAGGAA | GATGACTCTGTCGTTCGACG | Start: 115 |
| Tm | 58.519 | 58.474 | End: 269 |
| Size | 25 | 20 | 155 |
| Primer 3 details | |||
| Sequence | TGGTAGAAGCAAATCATACAAGGA | TCGTTCGACGATCTGCAAAT | Start: 115 |
| Tm | 57.939 | 58.009 | End: 259 |
| Size | 24 | 20 | 145 |
| Primer 4 details | |||
| Sequence | ATGGTAGAAGCAAATCATACAAGGA | GATGACTCTGTCGTTCGACG | Start: 114 |
| Tm | 58.337 | 58.474 | End: 269 |
| Size | 25 | 20 | 156 |
| Primer 5 details | |||
| Sequence | TTTGGAATTATTTCTGAAGGCACA | GATGACTCTGTCGTTCGACG | Start: 71 |
| Tm | 57.337 | 58.474 | End: 269 |
| Size | 24 | 20 | 199 |
