Primer Details for TRINITY_DN39753_c0_g1_i1 |
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| (TTA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGGGGCTTAATTCTATTTGTAGGA | ACATTTTTAATCCTCGGTAATGGTAT | Start: 152 |
| Tm | 59.611 | 57.01 | End: 259 |
| Size | 26 | 26 | 108 |
| Primer 2 details |
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| Sequence | TGAGGGGCTTAATTCTATTTGTAGG | ACATTTTTAATCCTCGGTAATGGTAT | Start: 152 |
| Tm | 58.399 | 57.01 | End: 259 |
| Size | 25 | 26 | 108 |
| Primer 3 details |
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| Sequence | AGGGGCTTAATTCTATTTGTAGGAGA | ACATTTTTAATCCTCGGTAATGGTAT | Start: 154 |
| Tm | 59.38 | 57.01 | End: 259 |
| Size | 26 | 26 | 106 |
| Primer 4 details |
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| Sequence | TCACTGACATTTATTGCAAGAACA | ACATTTTTAATCCTCGGTAATGGTAT | Start: 115 |
| Tm | 57.368 | 57.01 | End: 259 |
| Size | 24 | 26 | 145 |
| Primer 5 details |
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| Sequence | TGAGGGGCTTAATTCTATTTGTAGGA | ACATTTTTAATCCTCGGTAATGGTATT | Start: 152 |
| Tm | 59.611 | 57.584 | End: 259 |
| Size | 26 | 27 | 108 |
