Primer Details for TRINITY_DN3972_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTAGAACCCCCAGAACGGA | TCGAAACAATTTCAAATAAAACCTTGT | Start: 2 |
| Tm | 60.179 | 57.628 | End: 267 |
| Size | 20 | 27 | 266 |
| Primer 2 details |
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| Sequence | CTGTAGAACCCCCAGAACGG | TCGAAACAATTTCAAATAAAACCTTGT | Start: 1 |
| Tm | 59.751 | 57.628 | End: 267 |
| Size | 20 | 27 | 267 |
| Primer 3 details |
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| Sequence | GCTGTAGAACCCCCAGAACG | TCGAAACAATTTCAAATAAAACCTTGT | Start: 0 |
| Tm | 60.391 | 57.628 | End: 267 |
| Size | 20 | 27 | 268 |
| Primer 4 details |
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| Sequence | GTAGAACCCCCAGAACGGAG | TCGAAACAATTTCAAATAAAACCTTGT | Start: 3 |
| Tm | 59.464 | 57.628 | End: 267 |
| Size | 20 | 27 | 265 |
| Primer 5 details |
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| Sequence | TGTAGAACCCCCAGAACGGA | CGAAACAATTTCAAATAAAACCTTGTT | Start: 2 |
| Tm | 60.179 | 57.019 | End: 266 |
| Size | 20 | 27 | 265 |
