Primer Details for TRINITY_DN3958_c0_g1_i1 | |||
|---|---|---|---|
| (T)14: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACTATTCTTTTCACAGCAGCT | TCCTTGTTCTTCACACCTGCT | Start: 39 |
| Tm | 58.354 | 59.51 | End: 204 |
| Size | 23 | 21 | 166 |
| Primer 2 details | |||
| Sequence | TGACCTTTCCAAGATTCTGTTGT | TCCTTGTTCTTCACACCTGCT | Start: 98 |
| Tm | 58.208 | 59.51 | End: 204 |
| Size | 23 | 21 | 107 |
| Primer 3 details | |||
| Sequence | TGACTATTCTTTTCACAGCAGCT | CCTTGTTCTTCACACCTGCTC | Start: 39 |
| Tm | 58.354 | 59.124 | End: 203 |
| Size | 23 | 21 | 165 |
| Primer 4 details | |||
| Sequence | GTTGACTATTCTTTTCACAGCAGCT | TCCTTGTTCTTCACACCTGCT | Start: 37 |
| Tm | 60.049 | 59.51 | End: 204 |
| Size | 25 | 21 | 168 |
| Primer 5 details | |||
| Sequence | TGACTATTCTTTTCACAGCAGCTT | TCCTTGTTCTTCACACCTGCT | Start: 39 |
| Tm | 58.934 | 59.51 | End: 204 |
| Size | 24 | 21 | 166 |
