Primer Details for TRINITY_DN39584_c0_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACACAAGGAAACAGAAATTGAGA | TCATATGACTCACACGGCCA | Start: 65 |
| Tm | 57.831 | 58.809 | End: 164 |
| Size | 25 | 20 | 100 |
| Primer 2 details |
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| Sequence | AACACAAGGAAACAGAAATTGAGA | ATCATATGACTCACACGGCCA | Start: 66 |
| Tm | 57.226 | 59.238 | End: 165 |
| Size | 24 | 21 | 100 |
| Primer 3 details |
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| Sequence | TGAAAACACAAGGAAACAGAAATTGA | TCATATGACTCACACGGCCA | Start: 62 |
| Tm | 58.618 | 58.809 | End: 164 |
| Size | 26 | 20 | 103 |
| Primer 4 details |
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| Sequence | AGAAATAGTACGTTCAAACTCACCC | AAAAACATCCCCGACCTTCA | Start: 87 |
| Tm | 59.069 | 57.332 | End: 186 |
| Size | 25 | 20 | 100 |
| Primer 5 details |
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| Sequence | AAACACAAGGAAACAGAAATTGAGA | TCATATGACTCACACGGCCAA | Start: 65 |
| Tm | 57.831 | 59.447 | End: 164 |
| Size | 25 | 21 | 100 |
