ssr_primer
Primer Details for TRINITY_DN39559_c0_g1_i1 |
|---|
| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCAAAAGCAGGGGATGATGA | TGAGGGACTACAACATTGACGG | Start: 73 |
| Tm | 59.994 | 60.028 | End: 301 |
| Size | 21 | 22 | 229 |
| Primer 2 details |
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| Sequence | AGCAAAAGCAGGGGATGATGA | GAGGGACTACAACATTGACGGA | Start: 73 |
| Tm | 59.994 | 59.767 | End: 300 |
| Size | 21 | 22 | 228 |
| Primer 3 details |
|---|
| Sequence | AGCAAAAGCAGGGGATGATGA | AGGGACTACAACATTGACGGA | Start: 73 |
| Tm | 59.994 | 58.746 | End: 299 |
| Size | 21 | 21 | 227 |
| Primer 4 details |
|---|
| Sequence | GCAAAAGCAGGGGATGATGA | TGAGGGACTACAACATTGACGG | Start: 74 |
| Tm | 58.522 | 60.028 | End: 301 |
| Size | 20 | 22 | 228 |
| Primer 5 details |
|---|
| Sequence | AGCAAAAGCAGGGGATGATG | TGAGGGACTACAACATTGACGG | Start: 73 |
| Tm | 58.518 | 60.028 | End: 301 |
| Size | 20 | 22 | 229 |
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