Primer Details for TRINITY_DN39459_c0_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACGCGTATTAGATGTGCC | AGCTTACCTACTATTTGAACAGGA | Start: 120 |
| Tm | 59.141 | 57.131 | End: 221 |
| Size | 20 | 24 | 102 |
| Primer 2 details |
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| Sequence | CCACGCGTATTAGATGTGCC | AGCTTACCTACTATTTGAACAGGAAGA | Start: 120 |
| Tm | 59.141 | 59.82 | End: 221 |
| Size | 20 | 27 | 102 |
| Primer 3 details |
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| Sequence | CCACGCGTATTAGATGTGCC | AGCTTACCTACTATTTGAACAGGAA | Start: 120 |
| Tm | 59.141 | 57.746 | End: 221 |
| Size | 20 | 25 | 102 |
| Primer 4 details |
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| Sequence | CCACGCGTATTAGATGTGCC | GCTTACCTACTATTTGAACAGGAAGA | Start: 120 |
| Tm | 59.141 | 58.671 | End: 220 |
| Size | 20 | 26 | 101 |
| Primer 5 details |
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| Sequence | CCACGCGTATTAGATGTGCC | AGCTTACCTACTATTTGAACAGGAAG | Start: 120 |
| Tm | 59.141 | 58.668 | End: 221 |
| Size | 20 | 26 | 102 |
