Primer Details for TRINITY_DN3940_c1_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTGATAGGAAGATTGAGTTCTGA | ACACACACACACACACACAT | Start: 32 |
| Tm | 59.34 | 58.18 | End: 138 |
| Size | 25 | 20 | 107 |
| Primer 2 details |
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| Sequence | GCTGATAGGAAGATTGAGTTCTGAC | ACACACACACACACACACAT | Start: 33 |
| Tm | 59.247 | 58.18 | End: 138 |
| Size | 25 | 20 | 106 |
| Primer 3 details |
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| Sequence | GCTGATAGGAAGATTGAGTTCTGA | ACACACACACACACACACAT | Start: 33 |
| Tm | 58.088 | 58.18 | End: 138 |
| Size | 24 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | AGCTGATAGGAAGATTGAGTTCTG | ACACACACACACACACACAT | Start: 32 |
| Tm | 58.084 | 58.18 | End: 138 |
| Size | 24 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | AAGCTGATAGGAAGATTGAGTTCTGA | ACACACACACACACACACAT | Start: 31 |
| Tm | 59.844 | 58.18 | End: 138 |
| Size | 26 | 20 | 108 |
(TG)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTGATAGGAAGATTGAGTTCTGA | ACACACACACACACACACAT | Start: 32 |
| Tm | 59.34 | 58.18 | End: 138 |
| Size | 25 | 20 | 107 |
| Primer 2 details |
|---|
| Sequence | GCTGATAGGAAGATTGAGTTCTGAC | ACACACACACACACACACAT | Start: 33 |
| Tm | 59.247 | 58.18 | End: 138 |
| Size | 25 | 20 | 106 |
| Primer 3 details |
|---|
| Sequence | GCTGATAGGAAGATTGAGTTCTGA | ACACACACACACACACACAT | Start: 33 |
| Tm | 58.088 | 58.18 | End: 138 |
| Size | 24 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | AGCTGATAGGAAGATTGAGTTCTG | ACACACACACACACACACAT | Start: 32 |
| Tm | 58.084 | 58.18 | End: 138 |
| Size | 24 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | AAGCTGATAGGAAGATTGAGTTCTGA | ACACACACACACACACACAT | Start: 31 |
| Tm | 59.844 | 58.18 | End: 138 |
| Size | 26 | 20 | 108 |
