Primer Details for TRINITY_DN39339_c0_g1_i1 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCATCCAATGTGGGTTGGC | TTTTTCCCTATGTCAAGGTGTTTT | Start: 96 |
| Tm | 59.863 | 57.2 | End: 195 |
| Size | 21 | 24 | 100 |
| Primer 2 details | |||
| Sequence | CCAAAGCTACAAAGTCCGACG | TTTTTCCCTATGTCAAGGTGTTTT | Start: 62 |
| Tm | 59.54 | 57.2 | End: 195 |
| Size | 21 | 24 | 134 |
| Primer 3 details | |||
| Sequence | CAAAGCTACAAAGTCCGACGT | TTTTTCCCTATGTCAAGGTGTTTT | Start: 63 |
| Tm | 58.867 | 57.2 | End: 195 |
| Size | 21 | 24 | 133 |
| Primer 4 details | |||
| Sequence | CATCATCCAATGTGGGTTGGC | TTTTTCCCTATGTCAAGGTGTTTTA | Start: 96 |
| Tm | 59.863 | 57.1 | End: 195 |
| Size | 21 | 25 | 100 |
| Primer 5 details | |||
| Sequence | AGTGTCATCATCCAATGTGGGT | TTTTTCCCTATGTCAAGGTGTTTT | Start: 91 |
| Tm | 59.69 | 57.2 | End: 195 |
| Size | 22 | 24 | 105 |
