Primer Details for TRINITY_DN39275_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTGGGAAAGTCAAGAAACAGA | TGCTTTTTGTACACAAAGACTGGA | Start: 15 |
| Tm | 57.889 | 59.297 | End: 216 |
| Size | 23 | 24 | 202 |
| Primer 2 details | |||
| Sequence | TCTTGGGAAAGTCAAGAAACAGA | ACACAAAGACTGGAAAACATGTCT | Start: 15 |
| Tm | 57.889 | 59.05 | End: 206 |
| Size | 23 | 24 | 192 |
| Primer 3 details | |||
| Sequence | TCTTGGGAAAGTCAAGAAACAGA | TGCTTTTTGTACACAAAGACTGG | Start: 15 |
| Tm | 57.889 | 58.007 | End: 216 |
| Size | 23 | 23 | 202 |
| Primer 4 details | |||
| Sequence | TCTTGGGAAAGTCAAGAAACAGAA | TGCTTTTTGTACACAAAGACTGGA | Start: 15 |
| Tm | 58.494 | 59.297 | End: 216 |
| Size | 24 | 24 | 202 |
| Primer 5 details | |||
| Sequence | TCTTGGGAAAGTCAAGAAACAGA | TGCTTTTTGTACACAAAGACTGGAA | Start: 15 |
| Tm | 57.889 | 59.816 | End: 216 |
| Size | 23 | 25 | 202 |
