Primer Details for TRINITY_DN39163_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGTGCATGCATGTTCATGA | GCAGATTTGCTAATCATTGTCCT | Start: 104 |
| Tm | 60.179 | 57.489 | End: 204 |
| Size | 20 | 23 | 101 |
| Primer 2 details | |||
| Sequence | GCGTGCATGCATGTTCATGA | GCAGATTTGCTAATCATTGTCCTTG | Start: 104 |
| Tm | 60.179 | 59.253 | End: 204 |
| Size | 20 | 25 | 101 |
| Primer 3 details | |||
| Sequence | TGCGTGCATGCATGTTCATG | GCAGATTTGCTAATCATTGTCCT | Start: 103 |
| Tm | 60.457 | 57.489 | End: 204 |
| Size | 20 | 23 | 102 |
| Primer 4 details | |||
| Sequence | GCGTGCATGCATGTTCATGA | ACAATGCTCTAAACTTGTCCTACA | Start: 104 |
| Tm | 60.179 | 58.135 | End: 254 |
| Size | 20 | 24 | 151 |
| Primer 5 details | |||
| Sequence | GCGTGCATGCATGTTCATGA | GCAGATTTGCTAATCATTGTCCTT | Start: 104 |
| Tm | 60.179 | 58.101 | End: 204 |
| Size | 20 | 24 | 101 |
