ssr_primer
Primer Details for TRINITY_DN38825_c0_g1_i1 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGACCTCGAATGCAGTGAT | CGTCTCTTGTCTGAACCCCA | Start: 87 |
| Tm | 59.821 | 59.32 | End: 216 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
|---|
| Sequence | TTGAGGGACCTCGAATGCAG | CGTCTCTTGTCTGAACCCCA | Start: 83 |
| Tm | 59.75 | 59.32 | End: 216 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | ACCTCGAATGCAGTGATGCT | CGTCTCTTGTCTGAACCCCA | Start: 90 |
| Tm | 59.749 | 59.32 | End: 216 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | GGGACCTCGAATGCAGTGAT | CGTCCTTATCGAAATGGCCG | Start: 87 |
| Tm | 59.821 | 59.141 | End: 186 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | GCTGTCTTTGAGGGACCTCG | CGTCTCTTGTCTGAACCCCA | Start: 76 |
| Tm | 60.39 | 59.32 | End: 216 |
| Size | 20 | 20 | 141 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGACCTCGAATGCAGTGAT | CGTCTCTTGTCTGAACCCCA | Start: 87 |
| Tm | 59.821 | 59.32 | End: 216 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
|---|
| Sequence | TTGAGGGACCTCGAATGCAG | CGTCTCTTGTCTGAACCCCA | Start: 83 |
| Tm | 59.75 | 59.32 | End: 216 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | ACCTCGAATGCAGTGATGCT | CGTCTCTTGTCTGAACCCCA | Start: 90 |
| Tm | 59.749 | 59.32 | End: 216 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | GGGACCTCGAATGCAGTGAT | CGTCCTTATCGAAATGGCCG | Start: 87 |
| Tm | 59.821 | 59.141 | End: 186 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | GCTGTCTTTGAGGGACCTCG | CGTCTCTTGTCTGAACCCCA | Start: 76 |
| Tm | 60.39 | 59.32 | End: 216 |
| Size | 20 | 20 | 141 |
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