Primer Details for TRINITY_DN38544_c0_g1_i1 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACAAATTTTCACAAAACTCCATGT | ACCCATGATCCACCCACATG | Start: 50 |
| Tm | 58.898 | 59.74 | End: 159 |
| Size | 26 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | ACAAAGCAAGAATGACAAATTTTCACA | ACCCATGATCCACCCACATG | Start: 38 |
| Tm | 59.666 | 59.74 | End: 159 |
| Size | 27 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | TGACAAATTTTCACAAAACTCCATGT | GACCCATGATCCACCCACAT | Start: 50 |
| Tm | 58.898 | 59.447 | End: 160 |
| Size | 26 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | TGACAAATTTTCACAAAACTCCATGT | ATGACCCATGATCCACCCAC | Start: 50 |
| Tm | 58.898 | 59.447 | End: 162 |
| Size | 26 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | TTCACAAAACTCCATGTAAAAAGGA | ACCCATGATCCACCCACATG | Start: 59 |
| Tm | 57.589 | 59.74 | End: 159 |
| Size | 25 | 20 | 101 |
(AT)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACAAATTTTCACAAAACTCCATGT | ACCCATGATCCACCCACATG | Start: 50 |
| Tm | 58.898 | 59.74 | End: 159 |
| Size | 26 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | ACAAAGCAAGAATGACAAATTTTCACA | ACCCATGATCCACCCACATG | Start: 38 |
| Tm | 59.666 | 59.74 | End: 159 |
| Size | 27 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | TGACAAATTTTCACAAAACTCCATGT | GACCCATGATCCACCCACAT | Start: 50 |
| Tm | 58.898 | 59.447 | End: 160 |
| Size | 26 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | TGACAAATTTTCACAAAACTCCATGT | ATGACCCATGATCCACCCAC | Start: 50 |
| Tm | 58.898 | 59.447 | End: 162 |
| Size | 26 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | TTCACAAAACTCCATGTAAAAAGGA | ACCCATGATCCACCCACATG | Start: 59 |
| Tm | 57.589 | 59.74 | End: 159 |
| Size | 25 | 20 | 101 |
