Primer Details for TRINITY_DN38522_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGTCGCATGCAAACGATGG | AGCGATCTAAAAATAATGGCTGAA | Start: 73 |
| Tm | 59.901 | 57.057 | End: 245 |
| Size | 20 | 24 | 173 |
| Primer 2 details | |||
| Sequence | ATGTCGCATGCAAACGATGG | AGCGATCTAAAAATAATGGCTGAAA | Start: 73 |
| Tm | 59.901 | 57.666 | End: 245 |
| Size | 20 | 25 | 173 |
| Primer 3 details | |||
| Sequence | CGTGTCCGTTGCTTATGCTT | AGCGATCTAAAAATAATGGCTGAA | Start: 121 |
| Tm | 59.204 | 57.057 | End: 245 |
| Size | 20 | 24 | 125 |
| Primer 4 details | |||
| Sequence | GAAAATCCGTGTCCGTTGCT | AGCGATCTAAAAATAATGGCTGAA | Start: 114 |
| Tm | 59.128 | 57.057 | End: 245 |
| Size | 20 | 24 | 132 |
| Primer 5 details | |||
| Sequence | AGAAAATCCGTGTCCGTTGC | AGCGATCTAAAAATAATGGCTGAA | Start: 113 |
| Tm | 59.128 | 57.057 | End: 245 |
| Size | 20 | 24 | 133 |
