Primer Details for TRINITY_DN38450_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGCCCCAAGTGTTAACGG | CGTTGCTTCATTGTCATTTTTCTGT | Start: 121 |
| Tm | 60.251 | 59.54 | End: 230 |
| Size | 20 | 25 | 110 |
| Primer 2 details |
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| Sequence | GTGTTAACGGGTTTGGACGG | CGTTGCTTCATTGTCATTTTTCTGT | Start: 131 |
| Tm | 59.412 | 59.54 | End: 230 |
| Size | 20 | 25 | 100 |
| Primer 3 details |
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| Sequence | TGAGCCCCAAGTGTTAACGG | CGTTGCTTCATTGTCATTTTTCTG | Start: 121 |
| Tm | 60.251 | 58.103 | End: 230 |
| Size | 20 | 24 | 110 |
| Primer 4 details |
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| Sequence | GCCCCAAGTGTTAACGGGTT | CGTTGCTTCATTGTCATTTTTCTGT | Start: 124 |
| Tm | 60.825 | 59.54 | End: 230 |
| Size | 20 | 25 | 107 |
| Primer 5 details |
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| Sequence | TGAGCCCCAAGTGTTAACGG | GCTTCATTGTCATTTTTCTGTCAACC | Start: 121 |
| Tm | 60.251 | 60.074 | End: 226 |
| Size | 20 | 26 | 106 |
