Primer Details for TRINITY_DN38332_c0_g1_i1 | |||
|---|---|---|---|
| (TA)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTACTTTTGGTTTTGGCTTGT | GCTTTCTTTAATAGCAGAGGAGTGG | Start: 30 |
| Tm | 59.484 | 59.704 | End: 258 |
| Size | 23 | 25 | 229 |
| Primer 2 details | |||
| Sequence | TGGTACTTTTGGTTTTGGCTTGT | AGCAGAGGAGTGGTTATTGTTAA | Start: 30 |
| Tm | 59.484 | 57.501 | End: 246 |
| Size | 23 | 23 | 217 |
| Primer 3 details | |||
| Sequence | TGGTACTTTTGGTTTTGGCTTGT | ATAGCAGAGGAGTGGTTATTGTT | Start: 30 |
| Tm | 59.484 | 57.3 | End: 248 |
| Size | 23 | 23 | 219 |
| Primer 4 details | |||
| Sequence | TGGTACTTTTGGTTTTGGCTTGT | AATAGCAGAGGAGTGGTTATTGT | Start: 30 |
| Tm | 59.484 | 57.3 | End: 249 |
| Size | 23 | 23 | 220 |
| Primer 5 details | |||
| Sequence | TTGGTACTTTTGGTTTTGGCTTGT | GCTTTCTTTAATAGCAGAGGAGTGG | Start: 29 |
| Tm | 60.02 | 59.704 | End: 258 |
| Size | 24 | 25 | 230 |
