Primer Details for TRINITY_DN38085_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGAAACATTATTTAGGGTCAAGT | AGGTCTGGGAAATATCTTTCAATGA | Start: 162 |
| Tm | 58.199 | 58.026 | End: 323 |
| Size | 25 | 25 | 162 |
| Primer 2 details |
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| Sequence | AGGGAAACATTATTTAGGGTCAAGT | TGATGTTCTTAAAACTTGCCAAAAGT | Start: 162 |
| Tm | 58.199 | 58.673 | End: 291 |
| Size | 25 | 26 | 130 |
| Primer 3 details |
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| Sequence | AGGGAAACATTATTTAGGGTCAAGT | AGGTCTGGGAAATATCTTTCAATGAA | Start: 162 |
| Tm | 58.199 | 58.587 | End: 323 |
| Size | 25 | 26 | 162 |
| Primer 4 details |
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| Sequence | AGGGAAACATTATTTAGGGTCAAGT | AAGGTCTGGGAAATATCTTTCAATGA | Start: 162 |
| Tm | 58.199 | 58.587 | End: 324 |
| Size | 25 | 26 | 163 |
| Primer 5 details |
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| Sequence | AGGGAAACATTATTTAGGGTCAAGT | GGTCTGGGAAATATCTTTCAATGAA | Start: 162 |
| Tm | 58.199 | 57.355 | End: 322 |
| Size | 25 | 25 | 161 |
